REAGENT PREPERATION FOR Zn, Fe & Cu

           REAGENT PREPERATION FOR

 Zn, Fe & Cu in soil

DTPA

⬇

Take 1.967 gm AR DTPA and 1.470 gm CaCl2

⬇

               Add 25 ml Distilled Water

              ⬇

              Add 13.3 ml TEA

              ⬇

                Make up volume 1.0 Ltrs.

REAGENT PREPERATION FOR POTASSIUM

         REAGENT PREPERATION FOR POTASSIUM 

1.0 N Potassium Dichromate:

Dissolve 77.08 gm of Ammonium Acetate in 500 ml of D.W. and makeup Volume 1.0 Lts. Adjust the pH to 7.0 with Glacial acetic acid or Ammonium Solution.

Standard K Solutions:

Prepare 1000 mg L-1 K solution by dissolving 1.909 gm of dried (in oven) KCl salt per liter solution. Dilute volume of this a solution to get 100 ml working standards containing 10, 15, 20, 25, 30 and 40 mg KL-1. The working standards should be prepared in medium of extraction. 

REAGENT PREPARATION FOR DETERMINATION OF PHOSPHORUS IN SOIL

             REAGENT PREPARATION FOR DETERMINATION              OF PHOSPHORUS IN SOIL

0.5 NaHCO2 Solution:

       

Dissolve 42 gm of Sodium Bicarbonate in 500 ml of Hot Water and Dilute to 1.0 Lts. Adjust pH to 8.5 Using Dilute NaOH or HCl

1.5 % Ammonium Molybdate Solution:

Dissolve 15 gm of AR Ammonium molybdate in 300 ml of warm water, cool and exact 350 of 10  N HCl. Make the volume to 1.0 Lts.

40 % SnCl2 Stock Solution:

10 gm stannous chloride in a 100 ml of glass beaker. Add 25 ml of Conc. HCl and dissolve by heating. Cool and store in an umber coloured bottle in dark, after adding of a small piece of Zinc Metal AR to prevent oxidation 

 From Stock Solution prepare a dilute SnCl2 Solution

(.5 ml dilute to 66 ml in D.W.) Immediately before use.

REAGENT PREPERATION FOR DETERMINATION OF ORGANIC CARBON (Zn, Fe & Cu)

REAGENT PREPERATION FOR DETERMINATION OF  ORGANIC CARBON (Zn, Fe & Cu)

1.0 N Potassium Dichromate:
Dissolve 49.04 gm AR K₂ Cr₂ O₇  in 500 ml D.W. Make the Volume 1.0 Lts.

  0.5 N Ferrous Ammonium Sulphate:

Dissolve 196 gm Ferrous ammonium Sulphate in D.W

⬇

Add 20 ml of Conc. Sulfuric Acid and Make the Volume 1.0 Lts.

              Diphenylamine Indicator:

              Dissolve .05 gm Dye Mixture in 20 ml in D.W.  and 100 ml Conc.

                Sulfuric Acid  

DETERMINATION OF SULPHUR IN SOIL (Williams and Steinbergs, 1959 Method)

DETERMINATION OF SULPHUR IN SOIL

        (Williams and Steinbergs, 1959 Method)

Take 10.0 gm of Soil Sample in 100 ml of Conical Flask

⬇

Add 50 ml of Calcium Chloride of 0.15 %

⬇

Shake for 30 Minutes

⬇

Filter into 100 ml of Beaker through Whatman Paper No.1

⬇

Take 20 ml of clear filtrate into a 25 ml VF

⬇

Add 2.0 ml of Glacial Acetic Acid

⬇

Add 1.0 gm of Barium Chloride and shake

                               ⬇(Shake for 2 minutes)

Add 1.0 ml of 0.25 % Gum Acacia Solution

⬇

Add 1.0 ml of 0.25 % Gum Acacia Solution

⬇

Make Volume up to 25 ml with DW and Take reading at 420 nm wavelength by Spectrophotometer

  

DETERMINATION OF Zn, Fe, & Cu IN SOIL (Lindsay and Norvell,1978 Method)

       DETERMINATION OF Zn, Fe, & Cu IN SOIL

       (Lindsay and Norvell,1978 Method)

Procedure:

Take 10 gm of Soil Sample in 100 ml of Conical Flask

 ⬇

Add 20 ml Dietheline Triamine Penta Acetic Acid (DTPA) and Maintain pH 7.3 with HCL & NaOH

⬇

Shake for 2.30 Hours

⬇

Filter into 100 ml of Beaker through Whatman paper No. 41

⬇

Take Reading by Atomic Absorption Spectrophotometer (AAS) of the Filtrate

DETERMINATION OF POTASSIUM IN SOIL (Han Way and Heidel 1952 Method)

         DETERMINATION OF POTASSIUM IN SOIL 

       (Han Way and Heidel 1952 Method)

Procedure:

Take 5 gm of Soil Sample in 100 ml of Conical Flask

 ⬇

Add 25 ml of Ammonium Acetate of 1.0 Normality and Maintain pH 7.0 with Glacial Acetic Acid & Ammonium Solution

⬇

Shake for 30 Minutes

⬇

Filter into 100 ml Beaker through Whatman paper No.1

⬇

Take Reading by Flame Photometer of the Filtrate 

DETERMINATION OF PHOSPHORUS IN SOIL (Olsen’s Method 1954)

DETERMINATION OF PHOSPHORUS IN SOIL (Olsen’s Method 1954)

Take 2.5 gm of Soil Sample in 100 ml of Conical Flask

↓

Add Activate Charcoal 2 Spatula

↓

Add 50 ml of NaHCO3 0.5 M, Maintain 8.5 pH by HCl & NaOH

                                                                                          ↓     (Shake for 30 Minutes and Filter into

                                                                                     100 ml of Beaker through Whatma                                                                              Peper No.1 in 100 ml Beaker)

Take 5 ml Extract or Filtrate in 25 ml of Volumetric Flask

↓

Take 5 ml Extract or Filtrate in 25 ml of Volumetric Flask

↓

Add 5 ml Ammonium Molybdate, 1.5 % Solution

                                ↓(Remain Sample for 50 Minutes)

Make volume with D.W. Approx 22 ml

↓

Add Fresh 1.0 ml Stannous Chloride

↓

Make Volume up to 25 ml with DW and Take reading at 660 nm wavelength by Spectrophotometer

DETERMINATION OF ORGANIC CARBON IN SOIL (Walkly and Blank Method)

DETERMINATION OF ORGANIC CARBON IN SOIL   (Walkly and Blank Method)

Procedure:

• Take 1.0 gm of Soil Sample in 500 ml of Conical Flask
• Add 10 ml of Potassium Dicromate of 1.0 N
• Add 20 ml of Con. H­2­ ­­­_­–­­_­­­So 4
• Remain for 30 Minutes to Digestion
• Add 200 ml of Distilled Water 
• Add 10 ml of Orthophosphoric Acid of 85 %
• Add 1.0 ml of Diphinyle Indicator
• Titrate with  Ferrus Ammonium Sulfate of 0.5 N
• Note Reading Show on Burette 

DETERMINATION OF pH & EC IN SOIL

DETERMINATION OF pH & EC IN SOIL

Procedure:

•   Take 20 gm of Soil Sample into 100 ml of Beaker

•  Add 40 ml of Distilled Water

•  Shake for 30 Minutes 

•  Take Reading by pH Meter

•  For Electric Conductivity (EC) Determination: After getting pH reading, sample is allow to stand until clear supernatant liquid is obtained or  stand for over night and then take reading by EC meter.

Note :-  Before each determination, the electrode must be washed with distilled water and dried with the help of a piece of tissue paper.