DETERMINATION OF PHOSPHORUS
IN SOIL (Olsen’s Method 1954)
Take 2.5 gm of Soil Sample in
100 ml of Conical Flask
↓
Add Activate Charcoal 2
Spatula
↓
Add
50 ml of NaHCO3 0.5 M, Maintain 8.5 pH by HCl & NaOH
↓ (Shake for 30 Minutes and Filter into
100 ml
of Beaker through Whatma Peper No.1 in 100 ml Beaker)
Take
5 ml Extract or Filtrate in 25 ml of Volumetric Flask
↓
Take
5 ml Extract or Filtrate in 25 ml of Volumetric Flask
↓
Add 5 ml Ammonium Molybdate, 1.5 % Solution
↓(Remain Sample for 50 Minutes)
Make volume with D.W. Approx
22 ml
↓
Add Fresh 1.0 ml Stannous
Chloride
↓
Make Volume up to 25 ml with
DW and Take reading at 660 nm wavelength by Spectrophotometer
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