DETERMINATION OF SULPHUR IN SOIL (Williams and Steinbergs, 1959 Method)

DETERMINATION OF SULPHUR IN SOIL

        (Williams and Steinbergs, 1959 Method)

Take 10.0 gm of Soil Sample in 100 ml of Conical Flask

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Add 50 ml of Calcium Chloride of 0.15 %

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Shake for 30 Minutes

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Filter into 100 ml of Beaker through Whatman Paper No.1

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Take 20 ml of clear filtrate into a 25 ml VF

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Add 2.0 ml of Glacial Acetic Acid

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Add 1.0 gm of Barium Chloride and shake

                               ⬇(Shake for 2 minutes)

Add 1.0 ml of 0.25 % Gum Acacia Solution

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Add 1.0 ml of 0.25 % Gum Acacia Solution

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Make Volume up to 25 ml with DW and Take reading at 420 nm wavelength by Spectrophotometer