REAGENT PREPARATION FOR DETERMINATION OF PHOSPHORUS IN SOIL

             REAGENT PREPARATION FOR DETERMINATION              OF PHOSPHORUS IN SOIL

0.5 NaHCO2 Solution:

       

Dissolve 42 gm of Sodium Bicarbonate in 500 ml of Hot Water and Dilute to 1.0 Lts. Adjust pH to 8.5 Using Dilute NaOH or HCl

1.5 % Ammonium Molybdate Solution:

Dissolve 15 gm of AR Ammonium molybdate in 300 ml of warm water, cool and exact 350 of 10  N HCl. Make the volume to 1.0 Lts.

40 % SnCl2 Stock Solution:

10 gm stannous chloride in a 100 ml of glass beaker. Add 25 ml of Conc. HCl and dissolve by heating. Cool and store in an umber coloured bottle in dark, after adding of a small piece of Zinc Metal AR to prevent oxidation 

 From Stock Solution prepare a dilute SnCl2 Solution

(.5 ml dilute to 66 ml in D.W.) Immediately before use.